AB0095 By intravenous infusion marked by green fluorescent protein to reveal bone marrow mesenchymal stem cells’ distribution and differentiation of collagen induced arthritis rats
Background Rheumatoid arthritis (RA) is a autoimmune disease,which is characterized by the osteoclasia or the high deformity rate of cartilage and bone. According to some materials, Mesenchymal stem cells (MSCs) were definited as the cell full of proliferation, differentiation capacity, and potential immune regulation. MSCs transplantation could be a appropriate-designed pattern to the joint damaging from rheumatoid arthritis (RA).However, the repairing mechanism against osteoclasia of cartilage and bone is still unclear.
Objectives This study used collagen induced arthritis (CIA) rats as animal model to explore MSCs’ tissue repairing mechanism.
Methods We observed the ability of BMSCs differentiating into cartilage cells by toluidine blue staining in vitro;Then,BMSCs were labeled by the green fluorescent protein (GFP), infused into CIA through rats’ tail vein infusion. In different point time, the rat’s joints were made paraffin section,we observed the differentation of GFP positive cells and the distribution of GFP-positive cells differentiated chondrocytes by immunohistochemicale method.
Results First,we found BMSCs in vitro can differentiate into cartilage cells under a certain-culture condition. Then, BMSCs were labeled by the green fluorescent protein (GFP), infused into CIA through rats’ tail vein infusion. In different point time, the rat’s joints were made paraffin section,which the GFP positive cells were observed in synovium and bone marrow tissues after transplantation on the 3th day, and in cartilage tissues on the 11th day, then increased in cartilage tissues on the 30th day,42th day, by laser scanning confocal microscope. Anti-type II collagen, GFP double positive cells were found in articular cartilages (especially damaged part)by Anti-II collagen immunofluorescence technology.